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APC Anti-Mouse IL-4 Monoclonal Antibody 11B11 M100I9-11Cdatasheet
数据表
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概述
APC Anti-Mouse IL-4 Monoclonal Antibody
IL-4 is a pleiotropic cytokine produced by activated T cells, mast cells, and basophils. IL-4 is a potent lymphoid cell growth factor which stimulates the growth and activation of certain B cells and T cells. IL-4 is important for regulation of T helper subset development.
FCM
Mouse
Rat
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性能
liquid
Keep as concentrated solution. Store at 4°C and protected from prolonged exposure to light. Do not freeze.
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
0.2 mg/ml
11B11
Rat IgG1
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应用
Recommended Application: FC
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis (The amount of the reagent is suggested to be used ≤ 1.0 µg /10^6 cells in 100 µl). Since applications vary, the appropriate dilutions must be determined for individual use.
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图像
APC Anti-Mouse IL-4 Monoclonal Antibody.jpg
C57BL/6 mouse CD4+ splenocytes were stimulated with plate-bound anti-mouse CD3 in culture with anti-mouse CD28, IL-2 and IL-4 for 2 days, then followed by a 4-6 hour stimulation with PMA, ionomycin and golgi-stop. Then cells were stained with APC anti-mouse IL-4 (Figure A) and APC Rat IgG1 (Figure B) .
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