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Purified Anti-Human CD16 Monoclonal Antibody
CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct (CD16a and CD16b) forms. CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. It is over 95% homologous to CD16a in extracellular domain and is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex that functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).
FCM
Human
Liquid
Keep as concentrated solution. Store at 4°C as an undiluted liquid. For extended storage aliquot contents and freeze at -20°C or lower. Avoid cycles of freezing and thawing.
0.01M PBS pH 7.2 with 0.09%NaN3
Mouse IgG1
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis (The amount of the reagent is suggested to be used 0.25 µg per 100 µL of peripheral blood.). Since applications vary, the appropriate dilutions must be determined for individual use
Fcg RIII
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