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Anti Flag-tag mouse monoclonal antibody KM8002Ldatasheet
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数据表
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概述
Anti Flag-tag mouse monoclonal antibody
The DYKDDDDK peptide is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. As a member of Tag antibodies, Flag-tag antibody is the best quality antibody against DYKDDDDK in the research. Flag-tag antibody can recognize C-terminus, internal, and N-terminus Flag-tagged proteins.
WB,IF
A synthetic peptide DYKDDDDK coupled to KLH
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性能
Liquid
Store at -20°C or below. Avoid repeated freezing and thawing cycles
1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol
1 mg/ml
Mouse IgG
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应用
WB: 1:1000 ~ 1:10000, IF: 1:200 ~ 1:1000 Optimal dilutions/ concentrations should be determined by the end user
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图像
anti Flag-tag mouse mAb (2E4)
Western blot analysis of Flag-tag fusion protein with anti Flag-tag mouse mAb (2E4) diluted at 1: 2000 (lane1) and 1: 5000 (lane2).
anti Flag-tag mouse mAb (2E4)图2.jpg
ImmunoFluorescent analysis of the 293T cell transfected with anti Flag-tag mouse mAb (2E4). 1) Nuclei stained with DAPI; 2) ImmunoFluorescent analysis with c-Myc-tag; 3) Merge.
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