概述

产品名称：IF555 Goat anti-mouse IgG(H+L)

描述：Preparation: The polyclonal antibody was purified from goat antiserum by mouse IgG affinity chromatography. Specificity: The antibody reacts with whole molecule Mouse IgG. No antibody was detected against non-immumoglobulin serum proteins. The antibody may cross-react with immumoglobulins from other species.

应用范围：IF,FC

种属反应性：Mouse

性能

形态：Liquid

存储条件：Keep as concentrated solution. Store at 4°C and protected from prolonged exposure to light. Do not freeze.

存储溶液：PBS pH 7.2, 0.09% NaN3, 0.2% BSA

纯度：>90% by SDS-PAGE, 1.0 mg/ml

亚型：Polyclonal Goat IgG

应用

应用说明：FC: The amount of the reagent is suggested to be used ≤ 1 µg/10^6 cells. IF: 1/100 ~ 1/250 Since applications vary, the appropriate dilutions must be determined for individual use. iFluor™555 (IF555) has fluorescence excitation and emission maxima close to 555 nm and 570 nm. These spectral characteristics make it excellent alternatives to Cy3® and Alexa Fluor® 555.

图像

IF555 goat anti-mouse IgG Log Fluoresence Intensity

Human peripheral blood lymphocytes stained with purified mouse anti-human CD45 and IF555 goat anti-mouse IgG

IF555 goat anti-mouse IgG diluted

Immunofluorescence image of Anti-Flag monoclonal antibody stained HEK293 cell transfected with flag-tag fusion plasmid. The secondary antibody is IF555 goat anti-mouse IgG diluted at 1/250.