概述

产品名称：Protein marker 10-180 kDa

描述：SG2001 is a three-color protein standard with 10 prestained proteins covering a wide range molecular weights from 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). SG2001 is designed for monitoring protein separated during SDSpolyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.

应用范围：WB

应用

应用说明：Tested Applications: WB(Western Blotting), SDS-PAGE The application notes include recommended dilutions, the optimal dilutions/concentrations must be determined for individual use. For Western Blotting, use at an assay dependent concentration. ~1.5-2.5 μl per well for general Western transfering. Apply more for thicker (>1.5 mm) or larger gel. For SDS-PAGE, use at an assay dependent concentration. 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.

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Protein Ladder Data

SDS-PAGE Gel 1: Tris-Glycine (~4-20%), Gel 2: Bis-Tris (12%) MOPS buffer, Gel 3: Bis-Tris (10%) MES buffer. Pre-stained molecular weight standards have a differing mobility and as a consequence varying apparent molecular weight when run in distinct SDS-PAGE buffer systems.
The variance in pH of alternative buffers affects the charge of the labelled protein standard and its binding capacity for SDS. The apparent molecular weight of this marker has been determined by calibration against an unstained ladder in each electrophoresis condition.