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Anti His-tag mouse monoclonal antibody KM8001datasheet
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Anti His-tag mouse monoclonal antibody KM8001L
数据表
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概述
Anti His-tag mouse monoclonal antibody
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
WB,IF
A synthetic peptide HHHHHH coupled to KLH.
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性能
Liquid
Store at -20°C or below. Avoid repeated freezing and thawing cycles.
1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol
1 mg/ml
Mouse IgG
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应用
WB: 1:1000 ~ 1:10000, IF: 1:200 ~ 1:1000 Optimal dilutions/ concentrations should be determined by the end user.
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图像
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anti His-tag mouse mAb (5B8)
Western blot analysis of His-tag fusion protein with anti His-tag mouse mAb (5B8) diluted at 1: 2000(lane1) and 1: 5000(lane2).
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