概述

产品名称：FITC Anti-Mouse IL-17A Monoclonal Antibody

描述：IL-17A is a cystine-linked homodimeric pro-inflammatory cytokine produced by TH17 cells,a distinct CD4+ T cell lineage. IL-17A stimulates the production of the pro-inflammatory cytokines IL-1β, TNFα, and IL-6. IL-17A also induces production of the neutrophil chemoattractants IL-8, CXCL1, and CXCL6 thereby bridging adaptive and innate immunity . IL-17A is intimately involved in mucosal immunity against bacterial infections and has a putative role in some autoimmune disorders . IL-17A effects appear to be exerted primarily through binding to the IL-17RA.IL-17A binding induces production of cytokines, chemokines and other proteins through activation of the ERK1/2 MAP kinase, PI3K/Akt, p38, and NF-κB pathways . Phosphorylation of some Jaks and Stats has been observed.

应用范围：FCM

种属反应性：Mouse

免疫原：Mouse

性能

形态：liquid

存储条件：Keep as concentrated solution. Store at 4°C and protected from prolonged exposure to light. Do not freeze.

存储溶液：PBS pH 7.2, 0.09% NaN3, 0.2% BSA

纯度：0.5 mg/ml

克隆：17F3

亚型：Mouse IgG1

应用

应用说明：Recommended Application: FC

功能：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis (The amount of the reagent is suggested to be used ≤ 0.25 µg /10^6 cells in 100 µl). Since applications vary, the appropriate dilutions must be determined for individual use.

图像

FITC Anti-Mouse IL-17A Monoclonal Antibody.jpg

C57BL/6 mouse splenocytes were stimulated with plate-bound anti-mouse CD3 in culture with anti-mouse CD28, TGF-β and IL-6 for 2 days, then followed by a 4-6 hour stimulation with PMA, ionomycin and golgi-plug. Then cells were stained with PE anti-mouse CD4 and FITC anti-mouse IL-17A.