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Anti T7-tag mouse monoclonal antibody KM8010datasheet
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Anti His-tag mouse monoclonal antibody KM8001
数据表
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概述
Anti T7-tag mouse monoclonal antibody
The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.
WB
A synthetic peptide MASMTGGQQMG conjugated to KLH
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性能
Liquid
Store at -20°C or below. Avoid repeated freezing and thawing cycles.
1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol
1 mg/ml
Mouse IgG
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应用
WB: 1:1000~1:10000 Optimal dilutions/ concentrations should be determined by the end user.
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图像
Anti T7-tag mouse monoclonal antibody
Western blot analysis of T7-tag protein with anti T7-tag mouse mAb (5G10) diluted at 1: 5000, loading: 2.5 ng(left) and 1 ng(right).
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