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LE/AF Purified Anti-Mouse TIM-3 Monoclonal Antibody
CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on Th1 lymphocytes and CD11b+ macrophages. Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.
FCM
Mouse
Liquid
Keep as concentrated solution. Store at 4°C as an undiluted liquid. For extended storage aliquot contents and freeze at-20°C or lower. Avoid cycles of freezing and thawing.
PBS, pH 7.2
1mg/ml, >95% (by SDS-PAGE)
Rat IgG2a
This RMT3-23 antibody has been reported for use in flow cytometric staining. Additional reported applications (for relevant formats) include: in vitro and in vivo blocking of Tim-3, and immunohistochemical staining of frozen sections.
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