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PI/RNase Staining Solution
Description
Propidium Iodide (PI) is a fluorescent vital dye that stains DNA and RNA. PI binds to Product Information
both DNA and RNA, so the latter must be removed by digestion with ribonucleases. The
content of DNA as determined by flow cytometry, can reveal useful information about
the cell cycle and the proteins involved in the regulation of the cell cycle. Cells in G2 and
M phases of the cell cycle have double the DNA content of those in G0 and G1 phases.
Cells in S phase have a DNA content lying between these extremes. PI is detected in
the orange range of the spectrum using a 562-588 nm band pass filter. This reagent
may be used to analyze cell cycle by flow cytometry in addition to use with antibodies for
examining the expression of proteins during the cell cycle.
FC
Store at 4°C and protected from prolonged exposure to light. Do not freeze.
PBS pH 7.2, 0.09% NaN3
Flow cytometry