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Annexin V-FITC
Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. Annexin V Binding Buffer is recommended for use with Annexin V staining.
FC
Keep as concentrated solution.Store at 4°C and protected from prolonged exposure to light. Do not freeze.
PBS pH 7.2, 0.09% NaN3
Each lot of this reagent is quality control tested by immunofluorescent staining with flow cytometric analysis (The amount of the reagent is suggested to be used 5 µl/10^5 cells). Since applications vary, the appropriate dilutions must be determined for individual use.
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