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Preparation of monoclonal antibody
Technology application of Monoclonal antibody is the major innovation of immunological research. At present, preparation of monoclonal antibody is already very mature comparing to that of conventional polyclonal antibodies, which has stronger specificity, higher sensitivity, and facilitates large scale production; It has very obvious advantages. It has been widely used in various fields of scientific research, testing, medicine, etc.
1. Instructions:：
a. Immunogen can be recombinant proteins, natural proteins, synthetic peptide, small conjugate molecules.
b. The preparation times for standardized mono-antibody needs to be immunized for over three times.
c. The clients can increase the quantity of hybridoma cell lines, detailed information can be confirmed by phone.
d. Mono-antibody can be verified by Immunogen Elisa reaction, if it needs to be verified by other methods, please give a call to us.
2. Characteristics：
a. We have standardized, mature production system of monoclonal antibodies, the produced monoclonal antibodies can be comparable to well-known imported brands.
b. We have systematic detection platform of antibodies. We have stable testing platform of ELISA, WB, IP, IHC.
3. The client needs to provide the following substrates:：
a. Over 2mg proteins, purity should be over 85%, please provides infusion labeling proteins, screening proteins(purpose proteins with different tags).
b. If the antigen were polypeptide, please provide over 4mg polypeptide, purity should be over 90%.
c. If the antigen were small molecules, please provide over 5mg polypeptide, purity should be over 90%.

Services and prices

No. Project Period Products Price
KF3001 Preparation of standard mono-antibody 100 days Hybridoma cell lines（3 types） ¥ 25,100
Supernatant of hybridoma cell lines（1 ml/type ×3 types）
Experiment report
KF3002 Preparation and purification of standard mono-antibody 120 days Hybridoma cell lines（3 types） ¥30,000
Supernatant of hybridoma cell lines （1 ml/type ×3 types）
Purified antibody as client required in certain cell lines 5 mg
Experiment report
KF3003 Quick preparation of mono-antibody 45 days Hybridoma cell lines（1 types） ¥24,900
Supernatant of hybridoma cell lines （1 ml/type ×1 types）
Experiment report
KF3004 Quick preparation and purification of mono-antibody 65 days Hybridoma cell lines（3 types） ¥30,000
Supernatant of hybridoma cell lines （1 ml/type ×3 types）
Purified antibody as client required in certain cell lines 5 mg
Experiment report

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